Lysosomal membrane permeabilization (LMP) results in the release of cathepsins and other hydrolases from the lysosomal lumen into the cytosol, an important hallmark of lysosomal apoptosis and necrosis. CD BioSciences' expertise in lysosomes provides strong support for analysis of lysosomal membrane permeability. Our engineers develop a variety of strategies for evaluating LMPs to provide you with an efficient, cost-effective service that ensures your 100% satisfaction.
Background of Lysosomal Membrane Permeability
LMP is induced by a large number of different stimuli, including lysosomal promoters that have detergent-like effects or cause osmotic cleavage, reactive oxygen species (ROS) and members of the pro-apoptotic Bcl-2 family, which exert a pore-forming effect on the lysosomal membrane. Lysosomal hydrolases are released from the lysosomal lumen into the cytoplasmic lysate, and lysosomal histone proteases then act as executioners of subsequent cell death. Massive lysosomal leakage leads to increased cytosolic acidity, uncontrolled breakdown of cellular components and necrosis, ultimately leading to lysosome-dependent cell death. In anti-apoptotic cancer cells, lysosomal cell death has great potential in serving as a strategy to treat apoptosis deficiency in cancer.
Fig. 1. Lysosomes in the cell death pathway. (Wang F, et al., 2018)
Lysosomal Membrane Permeability Analysis Services
Lysosomes play a key role in cell death in both physiological and pathological settings, and a key feature of the lysosomal form of cell death is LMP. CD BioSciences focuses on how lysosomal membrane alterations lead to LMP. Our engineers are commmitted to developing multiple techniques for quantifying and visualizing LMP during the onset of death.
Our strategy allows for the detection of LMP onset and progression and the differentiation of partial LMP from total LMP. In addition, we use markers of mitochondrial outer membrane permeability (MOMP), plasma membrane instability, and autophagosome formation to provide additional information on whether LMP occurs upstream or downstream of cellular stress. Here, we combine the following strategies to provide comprehensive lysosomal membrane permeability analysis services to our global clients.
- Enzymatic Activity Measurements of Lysosomal Hydrolases in Cytoplasm
- Monitoring of Fluorescent Dextran Release from Lysosomes
- Detection of Cathepsins in Cytoplasm
- Analysis of the Lysosomal Galectin Puncta
Advantages of Our Services
- Various techniques to monitor LMPs, such as fluorescence microscopy, flow cytometry.
- Experienced lysosome research team.
- In combination with markers of other organelles and pathways that may be affected by lysosomal stress.
- Allows monitoring of the subset of lysosomes that display LMPs.
- The extent of LMP can be assessed.
- Specific signals released by lysosomes on LMPs can be assessed.
Our lysosomal membrane permeabilization service is widely used for the development of LMP-induced cell death as a therapeutic target for cancer. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
Reference
- Wang F, Gómez-Sintes R, Boya P. (2018) Lysosomal membrane permeabilization and cell death[J]. Traffic. 19(12): 918-931.