Lysosomes are important as a clearance organelle, but little is known about the mechanistic details of this pathway. In particular, little is known about the role of lysosomal protein degradation, mTOR signaling, and amino acid sensing. There is also growing evidence that lysosomal dysfunction plays a role in more common diseases, including inflammatory and autoimmune diseases, neurodegenerative diseases, cancer, and metabolic diseases. Advances in methodology have facilitated the development of isotopic labeling techniques, enabling the replacement of the corresponding element in a compound molecule with an isotope. It can be labeled into the drug molecule by chemical synthesis and followed by gas and liquid instruments. The development of this technology provides opportunities for research advances and drug discovery for targeted therapies against lysosomal proteins and biological processes.
Stable heavy isotopes of hydrogen, carbon and other elements have been incorporated into drug molecules, mainly as tracers for quantification during drug development. Among them, deuterium has gained our popularity due to its potential to influence the pharmacokinetic and metabolic characteristics of drugs. Our team of experts is dedicated to developing deuterium-labeled isotopes for lysosomal science research. In addition, we are developing other isotope-labeled products, including but not limited to：
❖ Radioisotope tracers, including 14C, 3H, 32P, 33P, 35S, 125I, 131I, etc. The selection of radioactive tracers will be based on several aspects such as experimental purpose, experimental period and operator safety, including the type of radiation, half-life, radiochemical purity, specific activity, toxicity and labeling location of the selected radioisotopes.
❖ Stable isotope tracers, mainly 2H, 13C, 15N, and 18O. The compounds produced using stable isotopes are either determined by the percentage of isotope labeling or by labeling specific atoms on the compound.
CD BioSciences is a distributor and manufacturer of high quality isotopically labeled scientific products. Our goal is to produce reliable isotope tracers for drug development against lysosomal diseases. We will perfect the technology of preparation and detection of isotope labeling reagents. We believe in bringing transparency and added value to our partners and customers. The markets served by our products include research and laboratories.
Applications of Isotope Labelings in Drug Development for Lysosomal Diseases
(1) Pharmacokinetic Studies
Radioisotope labeled compounds are used as tracers to study the distribution, metabolism, efficacy, and mechanism of action of drugs targeting lysosomal diseases in vivo, providing an important basis for pharmacokinetic studies of drugs, as well as providing methods for the development of innovative drugs.
(2) Toxicity Studies
Traditional toxicity studies mostly use in vitro and animal experiments on a large number of compounds, which require a lot of money and time, and cannot explain the mechanism of toxicity. The use of stable isotope labeled compounds allows the metabolic processes of drugs to be traced, the causes of toxicity to be identified, and the potential toxicity of new compounds to be predicted.
Advantages of Our Lysosomal Isotope Labelings
- High sensitivity to 1014-1018 g levels.
- Simple method, not interfered by other non-radioactive substances.
- Many complicated steps of substance separation can be omitted.
- The transfer and transformation of metabolites can be accurately quantified.
- In combination with certain morphological techniques, the quantitative distribution of radiotracer in lysosomes can be determined.
- Accurate localization of lysosomes can be achieved at the cellular level, subcellular level and even at the molecular level.
- Conform to physiological conditions.
CD BioSciences offers a wide range of professional lysosomal isotope labelings. Our aim is to provide the latest, economical and rapid isotope labelings to customers around the world. Over the years, we have continuously expanded our products to meet the needs of lysosomal science research. If you are interested in our products, please contact us for product quotation and more detailed information.