Lysosomes are dynamic organelles that act as regulatory hubs in cellular homeostasis. Changes in lysosome morphology, composition and turnover are often associated with disease. Therefore, rapid and efficient isolation of intact lysosomes is necessary to study their function and metabolites by proteomic analysis. However, the purification of lysosomes is a daunting task. This is mainly due to the heterogeneity of lysosomes, as well as their small number and high fragility. In addition, isolation methods must maintain the integrity of lysosomes while disrupting the plasma membrane, as their membrane rupture releases enzymes that can damage all organelles, including themselves. As an ideal partner for lysosomal research, CD BioSciences provides ultrapure and functional lysosome preparation services to customers worldwide.
Traditional methods for isolating lysosomes are based on density gradient ultracentrifugation. Large amounts of starting material are required and these methods are cumbersome and time consuming and contain significant cross-contamination. Working closely with cell biologists, CD BioSciences is committed to developing commercial kits for lysosome isolation, enabling fast and simple isolation and enrichment of intact lysosomes from crude cell and tissue extracts.
Our lysosome isolation kits provide a procedure for isolating lysosomes from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation. Sample types include walled cells, suspension cells and tissues.
Applications of Lysosome Isolation Kits
- Separation of cytosol and lysosomes.
- Obtain enriched light and heavy lysosomal fractions from cultured cells.
- Lysosome purification.
Downstream Applications of Isolated Lysosomes
- 2D/MS for proteomics studies.
- Electron microscopy.
- Disease profiling and gene expression.
- Signal transduction and interaction or localization.
Advantages of the Lysosome Isolation Kits
- Simple, fast and efficient.
- The number of starting cells/tissues required is much smaller than conventional methods.
- The entire protocol can be completed in less than 1.5 hours.
- Prepare samples for multiple downstream applications.
- Significantly enriches lysosomes in cultured cells or tissues.
- Come with complete instructions.
CD BioSciences offers a wide range of professional lysosome isolation kits. Our aim is to provide the latest, economical and rapid lysosome isolation kits to customers around the world. Over the years, we have continuously expanded our products to meet the needs of lysosomal science research. If you are interested in our products, please contact us for product quotation and more detailed information.