The nature of lysosomes as highly acidic, difficult-to-purify organelles and the fact that methods to purify them have remained largely unchanged over the years pose a significant challenge to lysosome research. CD BioSciences' expertise in lysosomes provides strong support for the purification of lysosomes from all cells. Our advanced technology platform will provide you with efficient, cost-effective services to ensure 100% satisfaction.
Introduction of Lysosomal Purification
With advances in biotechnology, studies have found that lysosomes are associated with many new proteins and key cellular functions. Some lysosomal diseases are caused by changes in lysosomal morphology, composition, and motility, which limit lysosomal enrichment schemes based on biophysical parameters. Furthermore, since lysosomes were discovered, methods for purifying them have remained largely unchanged, resulting in a somewhat hampered analysis of lysosomal function. However, organelle enrichment methods are crucial to facilitate the analysis of lysosomal biomolecules. Classical methods of purifying lysosomes mainly rely on separation of lysosomes by density gradient centrifugation.
Fig. 1. Quick and mild isolation of intact lysosomes using magnetic-plasmonic hybrid nanoparticles. (Le T S, et al., 2022)
Our Ultrapure and Functional Lysosomes Preparation Services
Organelle enrichment methods are essential to facilitate lysosomal biomolecular analysis. CD BioSciences is committed to providing customers worldwide with services for the preparation of ultra-pure and functional lysosomes. Our engineers have developed a complete protocol for isolating concentrated or purified lysosomal fractions from animal tissues and cultured cells, allowing highly functional and pure lysosomes to be obtained from small amounts of starting material and a variety of cells.
Here, we offer a variety of strategies to provide you with professional lysosome preparation services, as well as advanced techniques to determine the purity of lysosomal fractions. We will consider the advantages and disadvantages of the following strategies to develop the most cost-effective solution for you.
- Lysosome Purification by Gradient Density
- Lysosome Purification by Superparamagnetic Nanoparticles
- Lysosome Purification by Immunoprecipitation
- Lysosome Purification by Fluorescence-Assisted Organelle Sorting
- Lysosome Purification by Free Flow Electrophoresis
- Determination of the Purity of Lysosomal Fractions
Advantages of Our Services
- Lysosomal properties are fully considered to avoid interfering with the assay.
- Can be used for high yield lysosome purification and is compatible with histological analysis.
- Can be used to analyze lysosomal biomolecular composition and biological function.
- Achieves varying degrees of success in purifying lysosomes.
- Multiple strategies you can to choose.
- Advanced magnetic purification lysosome technology platform.
Our ultrapure and functional lysosome preparation services can help you obtain highly functional, high-purity lysosomes. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
Reference
- Le T S, Takahashi M, Isozumi N, et al. (2022) Quick and Mild Isolation of Intact Lysosomes Using Magnetic-Plasmonic Hybrid Nanoparticles[J]. ACS nano. 16(1): 885-896.