Lysosomal Electrophysiology Services

Introduction of Phagosome Acidification

The transport of H⁺ ions on the lysosomal membrane is the most widely studied, but many ion channels, including Na⁺, K⁺, Ca²⁺ and Cl^-, have been found in the intracellular lysosomal membrane. CD BioSciences' expertise in lysosomes provides strong support for analyzing the physiology and function of lysosomal ion channels. Our advanced lysosomal biology and electrophysiological technology platform will bring you efficient and economical services to ensure your 100% satisfaction.

Lysosomal Ion Channel

Intracellular ion channels located in various cellular organelles are fundamental to the control of many physiological functions. The function of the lysosome as a control center for cellular clearance is attributed to the activity of lysosomal ion channels and transport proteins, which maintain the concentration gradient of the lysosomal membrane by regulating ion influx and efflux. Lysosomal ion channels are thought to be the primary regulators of ion homeostasis maintaining the proper functioning of the lysosome. Importantly, structural or functional defects in these ion channels contribute to the development of lysosomal storage disease. The physiology and function of ion channels have been a hot topic of biomedical research. Patch clamping technique has been widely used to study the electrical properties of plasma membrane ion channels in various cells. However, their properties have been difficult to study due to the lack of direct Patch clamping methods for lysosomal membranes.

Fig. 1. Ion channels and ion transporters on the lysosomal membrane. (Xiong J, et al., 2016)

Our Services for Lysosomal Electrophysiology Analysis

Past studies of lysosomal channels forced to be expressed on the plasma membrane or reconstituted into lipid bilayers have mostly yielded inconclusive and conflicting results. For many years, CD BioSciences has dedicated itself to lysosomal electrophysiology studies to provide a comprehensive service for the physiological and functional analysis of lysosomal ion channels, including lysosomal K⁺ channels, lysosomal Ca²⁺ channels, and lysosomal Na⁺ channels. The lysosomal electrophysiology services we can provide include, but are not limited to:

• Localization Service of Lysosomal Ion Channels
• Analysis Service of Lysosomal Calcium Ion Channels
• Study of Lysosomal Channels Using Conventional Patch-Clamp Technique
• Study of Lysosomal Channels Using Lysosome Patch-Clamp Technique
• Measurement of Lysosomal Channels on Solid Supported Membranes
• Flux Measurement of Purified Lysosomes
• Nuclear Patch-Clamp Electrophysiology of Lysosomes
• Patch-Clamp Lysosomal Electrophysiology of Plant Vacuoles

We have already made significant progress in developing drugs that target lysosomal ion channels as a means of fighting disease. Here, our biologists use genetically encoded fluorescent probes and whole endolysosomal membrane clamp recording techniques to detect lysosomal channels under near physiological conditions. We can help you comprehensively examine the contribution of lysosomal ion channels to lysosomal function.

• Ion channel function and lysosomal degradation and autophagy.
• Maintenance of lysosomal membrane potential.
• Control of Ca²⁺ signaling.
• Promotion of cytokinesis.
• Lysosomal ion channels and transport proteins.

Advantages of Our Services

• Whole endolysosome recording provides direct analysis of lysosomal membranes under fairly simple recording conditions.
• New lysosomal channels and known channels can be easily identified and characterized.
• A unique approach can validate potential drugs targeting lysosomal channels and help find new strategies for treating lysosomal ion channel diseases.
• The location of lysosomal channels in their native environment can be measured directly.

Our professional services for lysosomal electrophysiology have been well received by customers. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.

Reference

1. Xiong J, Zhu MX. (2016) Regulation of lysosomal ion homeostasis by channels and transporters. Sci China Life Sci. 59(8):777-91.