Analysis Service of Lysosomal Calcium Ion Channels

Background of Lysosomal Calcium Ion Channels

Lysosomes are intracellular Ca²⁺ storage organelles that play an important role in a variety of membrane transport processes. As with the fusion of synaptic vesicles to the plasma membrane, the fusion of lysosomes to other membranes is specifically Ca²⁺ dependent. In addition, impairment of lysosomal Ca²⁺ homeostasis and membrane transport has been associated with many human diseases such as lysosomal storage disease (LSD), neurodegeneration, myopathy and cancer. Lysosomal membrane proteins, particularly ion channels, are essential for lysosomal Ca²⁺ signaling. Due to the intracellular localization of lysosomes and the lack of viable functional assays directly applicable to the natural environment, the role of lysosomal ion channels in Ca²⁺ signaling is less well understood than that of ion channels in the plasma membrane. The development of biomedical methods has made it possible to monitor lysosomal ion channels and their intracellular Ca²⁺ signaling.

Fig. 1. Schematic view of lysosomal Ca²⁺ signaling. (Zhong XZ, et al., 2017)

Services

Since lysosomal Ca²⁺ is indispensable for a variety of intracellular processes, and abnormal lysosomal Ca²⁺ homeostasis and defective lysosomal Ca²⁺ release are associated with many human diseases, it is important to monitor lysosomal Ca²⁺ content and lysosomal Ca²⁺ release. For many years, CD BioSciences has been dedicated to providing professional lysosomal Ca²⁺ channel analysis services for customers worldwide to assess channel-mediated lysosomal Ca²⁺ release and uptake via Ca²⁺ imaging. Our bioscientists employ a variety of strategies to analyze lysosomal Ca²⁺ ion channels.

Direct Monitoring of Ca²⁺ Levels in the Lysosomal Lumen

(1) Ca²⁺ Indicators

We select Ca²⁺ indicators with low sensitivity to pH to monitor lysosomal Ca²⁺ levels, such as Oregon Green 488, BAPTA-5N .

Selective Excretion of Lysosomal Ca²⁺ for Indirect Assessment of Lysosomal Ca²⁺ Content

(2) Small Molecule Fluorescent Ca²⁺ Indicator Dyes

We used Fura-2 to measure measure the relative lysosomal Ca²⁺ content, allowing quantitative measurement of cytoplasmic Ca²⁺ and GCamp or GECO adhering to the cytoplasmic surface of lysosomal membrane proteins.

(3) Genetically Encoded Ca²⁺ Indicators (GECIs)

We used GECIs to selectively detect lysosomal Ca²⁺ signals, allowing the study of lysosomal Ca²⁺ channel activity at high spatial and temporal resolution.

Why Choose Us

• Direct measurement of lysosomal Ca²⁺ channel activity can be measured.
• First-class laboratory equipment, proven technical support, lysosomal diaphragm clamp technology and GECIs.
• Measure specific lysosomal Ca²⁺ channels with higher spatial and temporal resolution.
• Expand understanding of lysosomal properties and lysosomal-related diseases.
• Standard scientific customized services.

CD BioSciences can meet any reasonable needs of our clients, taking time and budget into consideration for you. Our aim is to be customer-centric and to provide the highest quality services to customers. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.

Reference

1. Zhong XZ, Yang Y, et al. (2017) Methods for monitoring Ca²⁺ and ion channels in the lysosome. Cell Calcium. 64:20-28.