Lysosomes play a key role in cellular metabolism, endocytosis and synthesis/assembly of hydrolytic enzymes involved in macromolecular digestion. Lysosomes have an acidic inner lumen (pH 4.0-6.0) and contain a variety of proteases that are active at acidic pH. Currently, there are two main types of lysosomal markers, weakly basic amines (DAMP, neutral red, acridine orange) and LysoTracker probes, which selectively accumulate in lysosomes associated with acidic lumen pH. Nevertheless, these compounds still have defects. For example, DAMP is not fluorescent and additional fluorophores must be used to visualize lysosomal staining. In addition, neutral red and acridine orange, which normally stain acidic organelles, are not specific for lysosomes. elevated cell pH during prolonged intracellular accumulation of LysoTrackers may lead to quenching of the fluorochrome in addition to morphological/ physiological changes in lysosomes.
As an ideal partner for lysosome research, CD BioSciences is committed to developing lysosome staining kits that enable selective staining of lysosomes providing a powerful method for studying cellular events in spatial and temporal contexts.
CD BioSciences' lysosomal staining kits use a proprietary lysosomal dye that selectively accumulates in the lysosome through a lysosomal pH gradient. Soluble indicators are hydrophobic compounds that readily penetrate intact living cells and become trapped in the lysosomes upon entry, resulting in bright and specific lysosomal fluorescent staining. Lysosomal scramblers (e.g. chloroquine) are commonly used as positive controls to monitor changes in lysosome number and volume. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Applications of Lysosome Staining Kits
- Live cell imaging dyes are used to label lysosomes.
- For imaging the localization and morphology of lysosomes in living cells.
- High-inclusion analysis and screening applications
- For studies of cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity.
Advantages of Lysosome Staining Kits
- They are a set of fluorescent imaging tools.
- The dye accumulates in a low pH lysosomal environment resulting in highly specific lysosomal staining without the need for a washing step.
- Fluorescence is dependent on lysosomal pH changes.
- Require minimal hands-on time.
- Easily adapt to many types of fluorescence platforms, such as microplate assays, flow cytometry and fluorescence microscopy.
CD BioSciences offers a wide range of professional lysosomal staining kits. Our aim is to provide the latest, economical and rapid lysosomal staining kits to customers around the world. Over the years, we have continuously expanded our products to meet the needs of lysosomal science research. If you are interested in our products, please contact us for product quotation and more detailed information.