Background of Lysosomal Channels Study
Ion channels are located in the plasma membrane as well as in the mitochondrial, lysosomal and endoplasmic reticulum membranes. They play a key role in physiology and drug targeting. The patch-clamp technique is an electrophysiological method that allows the recording of whole-cell or single-channel currents flowing through ion channels across biological membranes. Using the patch-clamp, we can control and manipulate the voltage across the membrane or the whole cell to make high-resolution, low-noise measurements of ionic currents through the cell membrane. Patch-clamp technique has become the most powerful method for studying ion channel behavior, encompassing ion selectivity, channel dynamics and gating. Although membrane sheet clamp recordings have been widely used to study ion channels in the plasma membrane, ion channels in subcellular organelle membranes are challenging, including mitochondria, endoplasmic reticulum and lysosomes, because capillaries do not have access to organelle membranes.
Fig. 1. Illustration of whole-endolysosome patch-clamp recording. (Zhang X, et al., 2020)
Lysosomal Channel Study Service Based on Conventional Patch-Clamp Technique
Wild-type and mutant lysosomal ion channels can be targeted to the plasma membrane by re-expression so that they can be recorded in the plasma membrane using traditional patch-clamp techniques. CD BioSciences has established a proven platform for patch-clamp technology to monitor lysosomal ion channels. Here, our bioscientists offer customized services for studying lysosomal channels on plasma membranes or artificial membranes by the patch-clamp method, allowing the recording of a portion of the cell membrane or the entire cell by different configurations. The following mutated lysosomal ion channels that we monitor by this method are popular with our clients.
- Lysosomal TRPML1 channels
- Lysosomal TPC2 channels
- Lysosomal ClCs channels
In addition, we monitor the transport of ions across artificial membranes by reconstituting channel proteins into planar lipid bilayers in vitro. Our bioscientists develop customized processes for monitoring lysosomal TRPML1 channels.
Why Choose Us
- Extremely patient and highly skilled bioscientists perform manual manipulations.
- Provides high-resolution recordings of a portion of the cell membrane or the entire cell
- Records of excised cell patches with single or multiple ion channels are available.
- The behavior of single-molecule ion channels can be well controlled at the membrane layer.
- Can provide sufficient data throughput to record the current flowing through ion channels.
- Allows measurement of currents in ion channels on organelle membranes in a highly automated manner.
- Allows independent analysis of signal recording for ion channels with low probability of occurrence.
CD BioSciences can meet any reasonable needs of our clients, taking time and budget into consideration for you. Our aim is to be customer-centric and to provide the highest quality services to customers. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.
Reference
- Zhang X, Gu M, et al. (2020) Endosomal and Lysosomal Electrophysiology[M]//Ion and Molecule Transport in Lysosomes. CRC Press, 1-18.