Background of Lysosomal Proteome Dynamics Analysis
Lysosomes are highly dynamic degradative organelles that can move rapidly within the cell. The process of lysosomal dynamics regulation is a key element of lysosomal function. Lysosomes serve as regulatory centers for intracellular transport and platforms for intracellular signal transduction that depend on a complex set of interactions, including microtubule- and actin-based motors, protein complexes, and membrane contact sites between organelles in response to nutrient levels and lipid distribution in the membrane. However, the dynamic interactions of these molecules are not known. In addition, lysosomal dysfunction contributes to a variety of human diseases, such as lysosomal storage disorders, neurodegenerative diseases, metabolic diseases, and cancers. In these diseases, lysosomal proteins are significantly altered.
Fig. 1. Many mechanisms of lysosomal transport. (Cabukusta B, et al., 2018)
Our Lysosomal Proteome Dynamics Analysis Services
LSDs are a group of approximately 60 different disease conditions attributable to mutations in genes encoding proteins that play an important role in lysosomal function. These include lysosomal lumen and membrane proteins as well as non-lysosomal proteins involved in lysosomal function. In addition to providing lysosomal lumen and membrane protein identification services, CD BioSciences focuses on determining how lysosomal number, size, content and function vary across cell types and tissues, across individuals, and in response to different physiological and pathological conditions by using a histology-type approach.
Here, our engineers combine classical cell biology approaches with in vivo studies of transgenic mice under different environmental conditions and genetic manipulations to provide expert lysosomal proteome dynamics analysis services to our global clients. In addition, we identify DNA sequence variants in the genes encoding lysosomal proteins by whole genome and exome sequencing. In summary, our goal is to identify differences in lysosomal protein abundance due to altered cellular states or pathological conditions. We offer lysosomal proteome dynamics analysis services include, but not limited to:
- Analysis of Lysosomal Proteome Changes in Lysosomal Dysfunction Diseases
- Analysis of Lysosomal Proteome Changes in Microbial Infection
Why Choose Us
- Apply multi-omics types of methods for analysis, such as transcriptomics, proteomics, and lipidomics.
- Has the ability to modulate lysosomal function in a specific and targeted manner.
- The functional status of lysosomes can be monitored and determined in real time.
- Comprehensive analysis of the dynamics of specific lysosomal proteins in lysosomal dysfunction.
- Provides a revolutionary approach to the treatment of many devastating human diseases.
CD BioSciences can meet any reasonable needs of our clients, taking time and budget into consideration for you. Our aim is to be customer-centric and to provide the highest quality services to customers. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.
References
- Cabukusta B, Neefjes J. (2018) Mechanisms of lysosomal positioning and movement[J]. Traffic. 19(10): 761-769.
- Ballabio A, Bonifacino J S. (2020) Lysosomes as dynamic regulators of cell and organismal homeostasis[J]. Nature reviews Molecular cell biology. 21(2): 101-118.