Background of Lysosomal Quality Control During Autophagy
In eukaryotic cells, lysosomes are a very important intracellular degradation site, serving as the final destination during phagocytosis and autophagy while maintaining the cell's self-renewal capacity and energy supply. In addition, lysosomes also act as signaling hubs and play an important role in response to stressful stimuli. Despite being fortified with the glycocalyx, a 5-12 nm-thick layer of sugar residues on the luminal side of its membrane proteins, the lysosomal membrane remains susceptible to damage by various stressors. Lysosomal damage not only disrupts intracellular clearance but also results in the leakage of multiple contents, which pose great threats to the cell by triggering cell death pathways, including apoptosis, necroptosis, pyroptosis, and ferroptosis. Lysosomal quality control (LQC), including lysosomal repair, lysosomal phagocytosis, and lysosomal regeneration, is rapidly initiated in response to lysosomal damage to maintain lysosomal structural integrity and functional homeostasis. LQC may be a novel but critical target for disease therapy because of its integral role in maintaining cellular homeostasis and cell fate.
Fig. 1. Lysosomal quality control. (Shen H M, et al., 2014)
Services
Structural integrity and functional homeostasis of lysosomes are critical for cell function and viability. In response to various stimuli, endolysosomal damage leads to the formation of specific cellular components through effective LQC. For many years, CD BioSciences has been providing analytical services for lysosomal quality control during autophagy to clients worldwide, which is important for further exploration of effective lysosomal quality control (LQC)-based therapies. Our scientists have observed modifications on the luminal side of autophagy-promoting proteins and lysosomal membranes.
Here, we employ multiple strategies to analyze the lysosomal quality control process during autophagy.
Enhanced Lysosomal Membrane Repair
We identified and analyzed endosomal sorting complexes (ESCRT) that efficiently bind lysosomal membranes, enabling repair of small perforations in lysosomal membranes within minutes.
Enhanced Lysosomal Autophagy
We offer advanced techniques to identify and analyze proteins involved in damaged lysosomal ubiquitination, including galactose lectins (Gal3, Gal8 and Gal9) and Skp1/CUL1/F-box (SCF). We can quickly help you achieve selective autophagy initiation of lysosomes to ensure effective clearance of damaged lysosomes.
Enhanced Lysosome Regeneration
Our scientists combine bioinformatics and functional genomic analysis techniques to develop newly synthesized proteins that target lysosomes. We can combine TFEB with CLEAR elements to promote lysosomal renewal.
Why Choose Us
- Fully customizable service.
- Unique insight into lysosomal quality control from a team of professionals.
- Effective experimental design to quickly help you analyze and analyze the lysosomal quality control process during autophagy.
- Assist in drug development.
CD BioSciences can meet any reasonable needs of our clients, taking time and budget into consideration for you. Our aim is to be customer-centric and to provide the highest quality services to customers. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.
Reference
- Zhu SY, et al. (2020) Lysosomal quality control of cell fate: a novel therapeutic target for human diseases. Cell Death Dis. 11(9):817.