Analysis of the Function of Lysosomal Calcium in Cell Signaling

Cellular Ca²⁺ is a universal signal acting on a variety of physiological processes. Lysosomes are widely recognized as intracellular calcium stores that regulate autophagy and cancer progression by releasing calcium through some ion channels in the lysosomal membrane. CD BioSciences' expertise in lysosomes provides strong support for analyzing the interactions between Ca²⁺ signaling, lysosomes and the endoplasmic reticulum. Our advanced technology platform will bring you efficient and economical service, ensuring your 100% satisfaction.

Calcium in Cell Signaling Centers

Ca²⁺ is a key intracellular messenger that coordinates a variety of cellular functions such as fertilization, growth, differentiation and viability. Ca²⁺ signaling is regulated by a close association between intracellular organelles and plasma membrane channels, and organelle-specific Ca²⁺ processing is important for whole-cell Ca²⁺ homeostasis. In recent years, researchers have observed by electron cryomicroscopy the structure of endoplasmic reticulum (ER) Ca²⁺ channels, which are also a major source of intracellular calcium. In addition, the structure and physiopathological role of the mitochondrial calcium unidirectional transporter (MCU) complex is continuously updated, and the effect on lysosomal Ca²⁺ signaling is a milestone in the deeper understanding of the complexity of Ca²⁺ signaling.

Fig. 1. Major Ca²⁺ releasing channels and transporters on lysosomes. (Wu Y, et al., 2021)

Function of Lysosomal Calcium in Cell Signaling Analysis Services

Intramembrane interactions facilitated by scaffolding proteins play an important role in the formation and decoding of Ca²⁺ signals. For many years, CD BioSciences has worked to analyze the function of lysosomal calcium in cellular signaling. Our bioscientists develop customized fluorescence-based processes to detect Ca²⁺ signaling, ER and lysosomal-transport membranes to analyze their interactions.

1. We target fluorescent indicators to the lysosomal cytoplasmic surface to measure the local Ca²⁺ signal generated by the lysosome.
2. We designed Ca²⁺ sensors to target to the lysosomal lumen to reliably report the internal Ca²⁺ signal.

Our goal is to help you explore the potential role of lysosomal Ca²⁺ homeostasis in expanding the application of Ca²⁺ homeostasis in cancer development. Based on our advanced optical microscopy and technical platform of tagging endogenous proteins by gene editing, we can analyze the dynamic relationship between Ca²⁺ signals and organelles with precise spatial and temporal resolution. Here, we can provide services for the functional analysis of lysosomal calcium in cell signaling including but not limited to:

• Single-cell analysis of cytosolic Ca²⁺ signaling.
• High throughput analysis of cytosolic Ca²⁺ signaling
• Tracking lysosomal and ER interactions by fluorescence microscopy.

Advantages of Our Services

• Providing the resolution required to examine cell behavior at the most relevant temporal and spatial scales.
• Our genetically encoded fluorescent proteins enable subcellular targeting and probe optimization through directed evolution.
• Our fluorescent probes are designed to sense or manipulate Ca²⁺ with high sensitivity.
• Our fluorescent reporter genes allow for high-throughput analysis.

Our professional services for analysis of the function of lysosomal calcium in cell signaling have been well received by customers. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.

Reference

1. Wu Y, Huang P, Dong X P. (2021) Lysosomal calcium channels in autophagy and cancer[J]. Cancers. 13(6): 1299.