Rapid plasma membrane (PM) repair is critical for restoring cellular homeostasis and improving cell survival after injury. PM damage and rapid repair frequently occur in various environments. Lysosomal calcium-dependent exocytosis plays an important role in PM repair. CD BioSciences' expertise in lysosomes provides strong support for assessment of lysosomal-mediated PM repair responses. Our advanced advanced lysosome-mediated PM repair platform will bring you efficient and economical service, ensuring your 100% satisfaction.
Background of Lysosomal-Mediated Plasma Membrane Repair Responses
Maintenance of cellular homeostasis depends on the selective permeability of the PM. Therefore, restoration of PM integrity after injury plays an important role in maintaining eukaryotic cell integrity. PM damage triggers Ca2+ flux, and the damaged portion of the membrane is subsequently endocytosed by lysosomes to restore membrane integrity. One of the hallmarks of PM repair is the triggering of intracellular vesicle recruitment and extracellular secretion at the site of injury. Lysosomes serve as Ca2+-regulated secretory compartments, and some lysosomal enzymes are secreted extracellularly after PM permeability, playing a central role in membrane repair. PM repair defects have also been observed in cells carrying mutations that cause severe lysosomal disease.
Fig. 1. Evolutionary advantage of plasma membrane repair mediated by exocytosis of lysosomes. (Andrews NW, et al., 2018)
Lysosomal-Mediated Plasma Membrane Repair Responses Assessment Services
PM repair is a Ca2+-dependent process. The procedure to measure the degree of fusion of lysosomes with the PM of injured cells is an important indicator of cellular repair responses. CD BioSciences is dedicated to developing the extracellular roles of lysosomes and the specific functions of their numerous enzymes in PM repair. We focus on the following two aspects.
- Ca2+ regulated lysosomal exocytosis-mediated PM repair
The lysosomal synaptotagmin isoform Syt VII plays an important role in Ca2+-regulated plasma membrane repair. We inhibited lysosomal exocytosis by recombinant Syt VII C2 A domain or anti-Syt VII C2.
- Lysosome released protease-mediated PM repair
We analyzed the regulation of plasma membrane damage by lysosomal caspases and aspartyl proteases through enzyme inhibitors.
Here, our engineers developed multiple strategies to measure the degree of fusion of lysosomes with the PM of injured cells, including inducing wounds in different types of cells and sensitive assays that measure the cells' ability to secrete lysosomes and reseal their plasma membranes. Our lysosome-mediated PM repair response assessment services include, but are not limited to:
Advantages of Our Services
- Advanced lysosome-mediated PM repair platform.
- An efficient procedure to rapidly measure the degree of fusion of lysosomes with the plasma membrane of injured cells.
- Specificity of Enzyme Detection.
- Damages the plasma membrane in a dose-dependent manner.
- Lysosomal exocytosis can be quantified in whole cell populations.
- Provides a very useful tool to study the role of lysosomal exocytosis in plasma membrane repair.
Our lysosome-mediated PM repair response assessment service is widely used to study the role of lysosomes in plasma membrane repair. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
References
- Andrews NW, Corrotte M. (2018) Plasma membrane repair. Curr Biol. 28(8): R392-R397.
- Corrotte M, Castro-Gomes T, Koushik AB, Andrews NW. (2015) Approaches for plasma membrane wounding and assessment of lysosome-mediated repair responses. Methods Cell Biol. 126:139-58.