Phagosome maturation is the end of the phagocytosis process, during which internalized particles (such as bacteria and apoptotic cells) are transported into a series of acidified membrane-bound structures to fuse with acidic lysosomal structures, ultimately leading to degradation of internalized particles. Defective degradation will lead to a series of diseases. Thus, phagosome maturation plays an important role in maintaining normal homeostasis of the immune system, responses to bacterial pathogens, and even host immune responses to normal bacterial flora. However. To counteract acid-mediated defense mechanisms encountered at the extracellular or intracellular level, pathogens have evolved a range of strategies to survive harsh acidic conditions in the phagosome. Therefore, characterizing the physical correlates of phagosome maturation is critical for therapeutic design to understand the individual steps of cargo processing under phagocytosis.
Fig. 1. Mycobacterium tuberculosis controls phagosomal acidification by targeting CISH-mediated signaling. (Queval CJ, et al., 2017)
Phagosome Acidification Assessment Services
We provide biochemical and proteomic methods to identify proteins involved in phagosome maturation, helping you to analyze the different stages of phagosome maturation. Acidification is essential during phagosome maturation, and histone proteases will only degrade phagocytosed particles when phagosomes reach a low enough pH. Here at CD BioSciences, our biological scientists are committed to developing multiple strategies to assess the degree of phagosome acidification to analyze protein function during phagosome maturation. The services we can provide to assess the degree of phagosomal acidification include, but are not limited to:
In addition, we focus on the fact that there are many other steps in phagosome maturation, such as the accumulation of specific proteins on the phagosome. We analyze the phagosome maturation process by examining the functions of proteins involved in phagosome acidification, including ATPases, myosin motors, acid proteases, etc. We have established mature mammals and model organisms to determine the basic pathways of acidification of pathogen-containing phagosomes.
Why Choose Us
- Accessible lysosomal proteomics platform.
- Mature mammalian and model organism models.
- Comprehensive identification of genes and proteins associated with phagosomal maturation.
- Unbiased genetic screening approach.
- An essential tool for understanding bacterial microbial control of phagosome function and as a target for drug design.
CD BioSciences can meet any reasonable needs of our clients, taking time and budget into consideration for you. Our aim is to be customer-centric and to provide the highest quality services to customers. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.
Reference
- Queval CJ, Song OR, et al. (2017) Mycobacterium tuberculosis Controls Phagosomal Acidification by Targeting CISH-Mediated Signaling. Cell Rep. 20(13):3188-3198.