Effects of Cancer on Lysosome Compositiond
Normal lysosomal function is achieved through the action of two classes of proteins: lysosomal membrane proteins and lysosomal enzymes, mainly soluble lysosomal hydrolases. Approximately 50 lysosomal membrane proteins have been described. Soluble lysosomal hydrolases constitute the major degrading lysosomal component, and to date, more than 50 hydrolases and their auxiliary proteins have been detected. Lysosomal hydrolases include a variety of peptidases, nucleases, glycosidases, and sulfate lyases. These two classes of proteins are closely associated with cancer disease progression because they are involved in key processes leading to cell morphology, signaling, migration and invasion, and ultimately metastasis.
Fig. 1. Schematic representation of the components regulating the lysosomal nutrient sensing machinery upstream of mTORC1/TFEB/TFE3. (Machado E R, et al., 2021)
Services
Lysosomal membrane proteins and lysosomal enzymes are closely related to lysosomal function. Our aim is to analyze changes of lysosomal components (lysosomal membrane proteins and lysosomal enzymes) in cancer. At CD BioSciences, we are committed to providing a comprehensive analysis service of cancer-induced changes in lysosomal composition by detecting lysosomal proteins and lysosomal enzymes in cancer for our clients worldwide.
Changes in Lysosomal Hydrolases
- Aspartate histoproteinases (D and E).
- Serine histoproteinases (A and G).
- Asparagine endopeptidase.
- Cysteine histoproteinases (B, C, F, H, K, L, O, S, V, W and X/Z).
- Heparanase.
- Sialidase 1/Neuraminidase 1.
Changes in the Lysosomal Membrane Proteins
- Lysosomal Membrane Proteins LAMP1 and LAMP2.
- V-ATPase.
Based on the detection of lysosomal components in the cancers, we developed multiple cancer mouse models to assess the role of lysosomal proteins and lysosomal enzymes in cancer progression as follows:
- The role of lysosomal proteins and lysosomal enzymes in cancer cell signaling.
- The role of lysosomal proteins and lysosomal enzymes in invasion, migration and metastasis.
- The role of lysosomal proteins and lysosomal enzymes in cancer immune escape.
Our Available Strategies
Our skilled scientists develop platforms for quantitative histochemical and cytochemical methods that allow a direct link between lysosomal proteins and lysosomal enzymes and the morphology of tissues or cell types.
Cytofluorometric Method
We use a method based on naphthol AS-TR derivatives and the use of methoxynaphthylamine derivatives as substrates and 5'-nitro salicylaldehyde as coupling agent to analyze lysosomal histone protease activity. This method is more sensitive and capable of multiparameter analysis.
CD BioSciences can meet any reasonable needs of our clients, taking time and budget into consideration for you. Our aim is to be customer-centric and to provide the highest quality services to customers. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.
Reference
- Machado E R, et al. (2021) Lysosomes and cancer progression: A malignant liaison[J]. Frontiers in cell and developmental biology. 9: 642494.