Lysosomes are morphologically variable motile organelles, and in-depth study of lysosomal distribution and motility is essential for the development of new therapeutic approaches for needle lysosomal disruptive diseases. CD BioSciences' expertise prodides strong support for lysosomal motility determination service. Our advanced technology platform will bring you an efficient and cost-effective service that will ensure your 100% satisfaction.
Introduction of Lysosomal Motility
Lysosomes are dynamic organelles capable of fusing with multiple targets and reconstituting after a fusion event. Lysosomes play a major role in extracellular secretion, degradation of intracellular components and recycling. In addition, lysosomes interact with other intracellular organelles to regulate their turnover, such as the endoplasmic reticulum, mitochondria and peroxisomes. The proper localization of lysosomes is relevant to several physiological processes. Lysosomes containing undegraded substrates end up accumulating in the cell, thereby losing their localization and leading to lysosomal dysfunction, which affects a wide range of cellular functions. For understanding these functions and lysosomal interactions, their movement and cellular distribution are key. Currently, scientists are focused on lysosome distribution and motility and how autophagy is altered in these diseases . Therefore, we need to better understand the function, distribution, and motility of lysosomes.
Fig. 1. The location and function of lysosomes vary with different cell types. (Oyarzún J E, et al., 2019)
Our Lysosomal Motility Determination Services
Lysosomal distribution and motility are dramatically altered in a variety of lysosomal diseases. Quantification of lysosomal motility, direction of motion, displacement and velocity can be performed by fluorescence microscopy. To this end, CD BioSciences offers specialized lysosomal motility assay services based on an advanced imaging platform to determine lysosomal motility characteristics during cell lysis of tumor target cells. Here, we can help you analyze lysosomal motility in non-polarized cells, neurons and in vivo.
- Measurement of Lysosomal Motility in Non-Polarized Cells
Our engineers use Fiji's TrackMate plug-in to track lysosomal motion, a single-particle tracking software that allows segmentation of point-like or roughly spherical objects for each image over time. In addition, we use a different Gaussian (DOG) detector and a generic segmentation algorithm to track individual lysosomes. - Measurement of Lysosomal Motility in Neurons
Measuring lysosomal movement in neurons is challenging due to the size and differentiation of axons and dendrites. Our engineers quantified lysosomal motility, direction of movement, displacement and velocity in axons and dendrites in primary cultures of embryonic mouse or rat hippocampal or cortical neurons. - Analysis of Lysosomal Motility In Vivo
We have successfully established model systems of Drosophila melanogaster and zebrafish for in vivo studies. Our engineers have used genetically encoded fluorescent lysosomal markers on transgenic Drosophila for in vivo analysis of lysosomal morphology and motility.
Features of Our Services
- Independent open source software was developed to automatically classify trajectory and motion parameters for lysosomal transport analysis.
- Expression of fluorescently labeled lysosomes can be performed in virtually any cell type or tissue of interest.
- In vivo imaging of Drosophila lysosomes can be performed at each life cycle stage and combined with other manipulations, including genetics, pharmacology, or others.
- Our super-resolution microscopy technique can also analyze lysosomal trafficking in vivo.
Our lysosomal motility services provide a detailed solution protocol for lysosome distribution and kinetics. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
Reference
- Oyarzún J E, Lagos J, Vázquez M C, et al. (2019) Lysosome motility and distribution: Relevance in health and disease[J]. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 1865(6): 1076-1087.