Background of Light Microscopy-based Lysosome Monitoring
Lysosomes are small membrane-encapsulated cytoplasmic organelles ranging in size from 100 to 500 nm. Abnormalities and dysfunctions of lysosomes may lead to many pathologies. Therefore, developing a technique that can visualize and track lysosomes, as well as record the dynamics of lysosome movement in real time, are important for understanding how lysosomes work and the types of organelles they interact with. Light microscopy (LM), especially super-resolution microscopy (SRM), is a popular tool for monitoring cellular biological processes because it enables non-invasive real-time signals with high spatial and temporal resolution and sensitivity. LM has now been widely used to monitor the super-resolved structure of lysosomes in cells.
Fig. 1. Fluorescent probes for super-resolution microscopy of lysosomes. (Yadav A, et al., 2020)
Our Light Microscopy-based Lysosome Monitoring Services
Microscopic visualization is the first step in lysosomal analysis. As we all know, LM mainly realizes the monitoring of lysosomes by fluorescence. For many years, CD BioSciences has been working on developing new antibodies and dyes that selectively label lysosomes, providing promising tools for studying lysosomes. Here, we implement the observation of lysosomes by light microscopy in a different way.
- Immunolabeling of Lysosomal Proteins
This approach is performed mainly with antibodies against lysosomal membrane proteins (e.g., LAMP1 and LAMP2). The luminal lysosomal hydrolases are mainly labeled.
(1) We immunolabel antibodies against histone proteases. The premise is that these antibodies allow the visualization of lysosomes by immunofluorescence or immunohistochemistry. As well as relying on the use of fluorescently labeled lysosomal hydrolase inhibitors
(2) We visualized lysosomes by labeling transient or stable expression of lysosomal proteins.
- Visualization of Lysosomes by Endocytosis of Internalized Cargoes
We use a number of cargoes to localize lysosomes, such as dextran, fluorescently labeled epidermal growth factor (EGF) and fluorescently labeled bovine serum albumin (BSA).
- Observation of Lysosomes by Fluorescence Microscopy
This is a rapid and reliable method to infer possible pathological changes in lysosomes by studying the distribution, size and presence of accumulated luminal material.
We currently offer the following two strategies for monitoring lysosomes by light microscopy, which are well received by customers around the world. We will combine the advantages and disadvantages of the strategy to develop the most effective and cost-effective solution for you.
- Determination of lysosomal morphology by Dextran.
- Determination of lysosomal morphology by DQ-BSA.
Why Choose Us
- We implement 66+ antibodies suitable for immunofluorescence microscopy of fixed and permeabilized cells to observe lysosomes.
- Mature imaging technology platforms can resolve structures beyond the diffraction limit, including structured illumination microscopy, reversible saturated optical fluorescence transitions, and sub-diffraction resolution imaging.
- Multiple approaches can address the complex mechanisms that occur inside lysosomal structures that are smaller than the diffraction-limited point.
- New fluorescent probes can be designed for lysosomal dynamics in cells.
CD BioSciences can meet any reasonable needs of our clients, taking time and budget into consideration for you. Our aim is to be customer-centric and to provide the highest quality services to customers. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.
Reference
- Yadav A, Rao C, Nandi C K. (2020) Fluorescent probes for super-resolution microscopy of lysosomes[J]. ACS omega. 5(42): 26967-26977.