The autophagy-lysosome pathway plays an important role in maintaining cellular homeostasis by degrading large volumes of cytoplasmic material including damaged organelles and misfolded and accumulated proteins. Such accumulated proteins are common in neurodegenerative diseases (e.g. Parkinson's disease, Huntington's disease, and Alzheimer's disease) and not only lead to synaptic destruction and neuronal death, but also impair lysosomal function, thereby exacerbating their accumulation. Histone proteases, which are important performers of lysosomal degradation, are dysregulated in expression in neurodegenerative disease cells and they are less efficient in degrading misfolded proteins and dysregulated expression. Thus, a more comprehensive understanding of the common and unique features of lysosomal changes and dysfunction across the spectrum of neurodegeneration will help guide the development of therapeutic approaches to these devastating diseases.
Fig. 1. Lysosomal dysfunction in neurodegeneration. (Udayar V, et al., 2022)
Lysosomal Function Analysis Services in Aging-Related Neurodegenerative Disease
Genes that regulate lysosomal function are associated with common sporadic neurodegenerative diseases, and our scientists have attempted to develop them as targets for the treatment of neurodegenerative diseases. Here, CD BioSciences is committed to providing a comprehensive service for clients around the world to analyze lysosomal changes and dysfunction in aging-related neurodegenerative diseases, including Parkinson's disease (PD), Alzheimer's disease (AD), Huntington's disease (HD), amyotrophic lateral sclerosis (ALS), etc. Our proven organoid models and advanced methodological platforms will ensure your 100% satisfaction.
We offer a variety of disease models to analyze lysosomal genetic variants in neurodegenerative diseases.
- In vivo mouse models of neurodegenerative diseases.
- Human iPSC-derived neurons, microglia, macrophages and astrocytes from patient fibroblasts, which adequately recapitulate disease-like phenotypes at the cellular and molecular levels.
Lysosomal genes and the pathways they affect are an element of the underlying pathophysiological processes in neurodegenerative diseases. Our experienced team developed the following strategies to neurodegeneration related to the specific role of lysosomal genes.
- Screening to identify genetic modifiers of disease
We identify genetic modifiers of neurodegenerative diseases to determine the impact of risk alleles in lysosomal genes on the disease. Our solution allows for large-scale CRISPR-based genetic screens to identify modifiers that affect lysosomal function or regulate the activity of specific lysosomal enzymes. The methods we use include:- Family studies.
- Large-scale association studies.
- Genome sequencing of biological models with a wide range of different phenotypes.
- Next-generation translational research in lysosome-associated neurodegenerative diseases
We offer single-cell RNA sequencing and single-cell proteomics to analyze specific individual cell types in diseases carrying risk alleles in lysosomal genes. This can quickly help you develop emerging lysosomal biomarkers associated with neurodegenerative diseases.
Advantages of Our Services
- Mature human disease-associated induced pluripotent stem cell models.
- Advanced methods such as clustered regularly interspaced short palindromic repeat sequences (CRISPR), single-cell genomics and deep sequencing.
- Comprehensive, more fine-grained analysis services.
- Explore new identified therapeutic targets for lysosomal-related diseases.
Our professional services for the analysis of lysosomal changes and dysfunction in aging-related neurodegenerative diseases have been well received by customers. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
Reference
- Udayar V, et al. (2022) Lysosomal dysfunction in neurodegeneration: emerging concepts and methods[J]. Trends in Neurosciences.