Autoimmune diseases occur when the body's natural defense system is unable to distinguish between its own cells and foreign cells, causing the body to mistakenly attack normal cells. To date, more than 80 autoimmune diseases affect people's lives. Lysosomes are involved in core pathways of the immune system, including degradation of intracellular and extracellular substances, plasma membrane repair, cell death signaling, cellular homeostasis and death. Therefore, lysosomes are important for normal immune system function. It has long been anticipated that lysosomal dysfunction would have a major impact on immune diseases, yet this area has not been extensively explored. The role of lysosomes will continue to be of increasing interest in immune research, and a comprehensive understanding of the role of lysosomes in immune diseases will help provide new molecular targets and therapeutic strategies for disease treatment.
Fig. 1. Alterations of lysosomal functions in autoimmune diseases. (Wang F, et al., 2015)
Services
CD BioSciences is committed to providing our global clients with a comprehensive service to analyze lysosomal changes and dysfunction in autoimmune diseases by detecting autophagy, tissue protease expression, and luminal pH. Our scientists build animal models of autoimmune disease and obtain immune cells to analyze the critical role of lysosomes in a number of common autoimmune diseases, including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), dermatomyositis, and psoriasis.
Lysosomal Cathepsin
We analyzed the expression and activity of cathepsins S and L in autoimmune diseases. We provide a quick and simple method to measure the activity of lysosomal cathepsins S and L present in dried body fluids and cellular tissue samples. Furthermore, we combined multi-omics techniques to characterize the deregulated expression of lysosomal proteases in autoimmune diseases.
Lysosomal pH
Most lysosomal proteases exhibit optimal activity in the acidic environment of the lysosome (pH 4.0-5.0). We developed specialized scaled fluorescent dyes and fluorescent probes to measure lysosomal pH in lupus B cells.
Lysosomal Autophagic Pathway
Different cell subpopulations in different autoimmune diseases exhibit a variety of autophagic alterations. Our scientists carefully designed and tested interventions targeting the autophagic pathway, and developed mouse models lacking LC3-related phagocytosis to assess lysosomal autophagic changes in autoimmune diseases.
Our Available Technologies
- Transmission Electron Microscopy
- Multi-Omics Techniques
- Gene Knockdown Techniques
- Pharmacological Methods
Advantages of Our Services
- Multiple cutting-edge technologies.
- Convenient and efficient probe for lysosomal pH measurement.
- Team with expertise, working with international experts.
- A wide selection of in vivo and in vitro models of autoimmune diseases.
- Facilitates the design of innovative autophagy-targeted therapies.
Our professional services for the analysis of lysosomal changes and dysfunction in autoimmune diseases have been well received by customers. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
Reference
- Wang F, Muller S. (2015) Manipulating autophagic processes in autoimmune diseases: a special focus on modulating chaperone-mediated autophagy, an emerging therapeutic target[J]. Frontiers in immunology. 6: 252.