Cardiovascular disease, a group of diseases that affect the function of the heart and blood vessels, is currently the leading cause of death worldwide, claiming more lives than all forms of cancer combined. Atherosclerosis is a major contributor to this burden and underlies all forms of cardiovascular disease, including peripheral arterial disease, myocardial infarction and stroke. Many cardiovascular diseases are thought to be closely associated with lysosomal alterations and dysfunction. One major type of lysosomal dysfunction leading to cardiovascular disease is caused by deficiencies in lysosomal enzymes, membrane transport proteins, or other proteins related to lysosomal biology. In addition to lysosomal components, the regulation of lysosomal biosynthesis is also closely associated with the development of cardiovascular disease. Therefore, exploring the role and regulation of lysosomal function in the onset and progression of vascular disease may provide new insights into the understanding of vascular pathobiology and contribute to the development of more effective therapeutic strategies for cardiovascular disease.
Fig. 1. Role of lysosomes in development of atherosclerosis. (Zhang Z, et al., 2021)
Services
CD BioSciences is committed to providing our global clients with a comprehensive service to analyze lysosomal changes and dysfunction in cardiovascular disease through the detection of lysosomal enzymes, membrane transport proteins and autophagic fluxes. Our mission is to help you characterize the multiple processes that lead to impaired lysosomal homeostasis in cells with cardiovascular disease.
Our skilled scientists build multiple animal models of cardiovascular disease and multiple cellular models that take into account the heterocytic nature of the heart to analyze defective lysosomal function.
Lysosomal Function in Atherosclerosis Model
We use a cell culture model of atherosclerosis to analyze the increase in lysosomal pH, lysosomal protein hydrolysis capacity in macrophages with high levels of oxidized LDL and cholesterol crystals.
Lysosomal Function in Transgenic Mouse Model
We constructed macrophage-specific TFEB overexpressing transgenic mice to analyze lysosomal biosynthesis and protein hydrolysis in macrophages with atherogenic lipids.
Lysosomal Function in Ischemia/Reperfusion (IR) Injury Model
We analyzed lysosomal-autophagic pathway function in a mouse model of ischemia/reperfusion (IR) injury and in human cardiac macrophages with ischemic cardiomyopathy.
Lysosomal Function in Cardiotoxicity Model
We examined the importance of the lysosomal-autophagic pathway in cardiomyocytes in a model of adriamycin (DOX)-induced cardiotoxicity, focusing on the analysis of lysosome numbers, histone activity and autophagy in cardiomyocytes.
Lysosomal Function in Ex Vivo Model of Nutrient Overload
We used an ex vivo model of nutrient overload to analyze the effects of saturated fatty acid palmitate on cellular TFEB levels, lysosome content, lysosomal proteolytic capacity, and lysosomal autophagy.
Our Available Technologies
- A hiPSC-Based Disease Modeling System
- CRISPR/Cas9 Genome Editing Technology
- Multi-Omics Techniques
- Pharmacological Methods
Advantages of Our Services
- Multiple cutting-edge technologies.
- Simple and fast experimental process to deliver results at an economical cost.
- Team with expertise and collaboration with international experts.
- Extensive and proven models of cardiovascular disease.
- Increased understanding of how dysregulation of lysosomal pathways in the cardiovascular system leads to related diseases.
Our professional services for the analysis of lysosomal changes and dysfunction in cardiovascular diseases have been well received by customers. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
Reference
- Zhang Z, et al. (2021) Role of lysosomes in physiological activities, diseases, and therapy[J]. Journal of Hematology & Oncology. 14(1): 1-39.