Autophagy is a highly conserved mechanism for delivering cytoplasmic components for lysosomal degradation. Among them, chaperone-mediated autophagy (CMA) is the first study to show that lysosomal degradation of intracellular components can be a selective process. CD BioSciences' expertise in lysosomes provides strong support for the study of lysosomal biology in chaperone-mediated autophagy. Our advanced systems biology computational analysis platform can complete lysosomal biology analysis in autophagy to ensure your 100% satisfaction.
Lysosome and Chaperone-Mediated Autophagy
CMA is a protein hydrolysis system that contributes to intracellular protein degradation in lysosomes. Molecular chaperones in the cytosol and lysosomal lumen stimulate this protein hydrolysis pathway. Lysosomes can degrade the cell cytoplasm in a non-specific manner, or they can differentiate between degradation targets by degrading tags, chaperones and a complex mechanism that allows selected proteins to cross the lysosomal membrane through specialized translocation complexes. Using transgenic mouse models to study the regulation of CMA activity in vivo, scientists have found that selectively conferring the ability to engage CMA in the regulation of multiple cellular functions. the timely degradation of specific cellular proteins by CMA can be regulated, as well as cellular quality control through the removal of damaged or faulty proteins. CMA dysfunction has also been extensively studied in relation to a variety of pathologies.
Fig. 1. Main physiological roles of CMA. (Kaushik S, et al., 2018)
Services
CMA is a selective autophagy for lysosomal degradation of proteins with KFERQ peptide motifs. CD BioSciences is committed to providing comprehensive research services on lysosomal biology in chaperone protein-mediated autophagy to clients worldwide. Our goal is to help you analyze the molecular dynamics, regulation and physiology of CMA in a simple way. We have successfully constructed transgenic mammalian and avian model platforms to study chaperone-mediated autophagy.
CMA Report
- Pulse tracking experiments.
- CMA activity measurement of intact cells in culture.
- Unrestricted sample size for testing.
Lysosomal Uptake
- In vitro assay.
- Direct measurement of CMA activity.
- Binding and uptake can be measured separately.
Lysosomal Protein Degradation
- In vitro assay.
- Direct measurement of CMA activity.
Kinetics of CMA translocation complexes
- In vitro assay.
- Provides information on CMA dynamics.
- Used to identify possible failures in the CMA step.
CMA Degrades Proteins
- Possible compensatory changes.
- Evaluates other forms of autophagy in the same experiment.
Immunofluorescence
- Measures the number of lysosomes performing CMA.
- Useful decisions.
Our scientists reconstruct in vitro the optimized system of CMA to identify chaperone-cargo and substrate lysosomal translocations. Our research services for lysosomal biology in chaperone-mediated autophagy include:
- Identification of Key Chaperone-Mediated Autophagy Molecules
- Analysis of LAMP-2A Dynamics
- Assessment of Chaperone-Mediated Autophagy Activity
Our Advanced Technical Support
- Mammalian RNAi Technology
- Transgenic Mouse Model
- Biochemical and Genetic Methods
- High-Resolution Technology
Our professional services for the study of lysosomal biology in chaperone-mediated autophagy have been well received by customers. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
Reference
- Kaushik S, Cuervo A M. (2018) The coming of age of chaperone-mediated autophagy[J]. Nature reviews Molecular cell biology. 19(6): 365-381.