Background of Lysosomal Lipidome Quantification
The cellular lipidome consists of a large number of unique structures containing hundreds of thousands of different lipid molecular species. Cellular lipid catabolism occurs primarily in lysosomes and closely related late endosomes. Among the more than 60 soluble hydrolases in the lumen of lysosomes are many lipases, such as phospholipase, acid sphingomyelinase, acid ceramic enzyme, hexosaminidase, galactosidase, and arylsulfatase. Their main function is to assist the recycling of plasma membranes and intracellular organelles by hydrolyzing lipids, providing the basis for lipid biosynthesis. In addition, lysosomal lipid catabolism can provide cells with an additional energy source. In conclusion, lipids play key roles in lysosomal function.
Fig. 1. Schematic diagram of functional lipidomics. (Han X, et al., 2021)
Our Lysosomal Lipidome Quantification Analysis Services
Lysosomal lipidomics provides detailed information about lysosomes and helps to gain insight into the possible origin, structure and functional alterations of lysosomes. Based on obtaining high purity lysosomes, we extract and isolate lysosomal lipids through a variety of techniques. CD BioSciences is committed to addressing the complexity of the lysosomal lipidome through new technologies, robotic fluid handling systems and spatial reconstruction methods to quantify hundreds of lipids with different physicochemical properties.
Our engineers have developed detailed protocols for the quantitative analysis of the lysosomal lipidome. We can help you extract a broader range of lipids and classify them according to their physicochemical properties to support the resolution of a large number of lipid species in a mass spectrometer. Here, we combine superparamagnetic colloidal iron dextran (FeDEX) particles loaded lysosomal and birdshot lipidomics to quantitatively monitor hundreds of lipid species. The protocol flow is as follows:
(1) FeDEX is added to the cell culture medium.
(2) FeDEX is taken up by cells via the endocytic pathway and eventually accumulates in the lysosomal compartment.
(3) The FeDEX-loaded lysosomes are specifically captured on a magnetic column after mild plasma membrane cleavage.
(4) Mass spectrometry-based lipid analysis.
Why Choose Us
- Powerful, automated, and sensitive birdshot-based high-throughput platform for lipidomics.
- Fast and efficient detection procedure.
- Easily adaptable to any laboratory.
- All-round lipid identification and quantification directly from crude lipid extracts of any biological material.
- Eliminates the need for upstream chromatographic separation of lipid samples.
- Fully automated sampling allows processing of large volumes of samples.
CD BioSciences can meet any reasonable needs of our clients, taking time and budget into consideration for you. Our aim is to be customer-centric and to provide the highest quality services to customers. Our customer service representatives are enthusiastic and trustworthy 24 hours a day, 7 days a week. If you are interested in our services, please feel free to contact us for more information or a detailed discussion.
References
- Bilgin M, Nylandsted J, Jäättelä M, et al. (2017) Quantitative profiling of lysosomal lipidome by shotgun lipidomics[M]//Lysosomes. Humana Press, New York, NY. 19-34.
- Han X, Gross R W. (2021) The foundations and development of lipidomics[J]. Journal of lipid research. 100164.