RNA interference (RNAi) is a conserved endogenous activity that serves as a tool for functional genomics studies. This method is widely used for large-scale identification of novel lysosomal transport proteins involved in vesicle and cargo transport to study lysosomal function in mammalian cells. CD BioSciences' expertise in lysosomes provides strong support for RNAi screens of lysosomal trafficking. Our advanced RNAi high-throughput screening platform will bring you efficient and economical service, ensuring your 100% satisfaction.
Background of RNAi Screens of Lysosomal Trafficking
RNAi technology is the introduction of gene-specific agents into cells that trigger reduced gene function through sequence-specific degradation of mRNA transcripts and translational interference. Therefore, this method can be used for large-scale screening to interrogate the functions of various model organisms and a growing number of other species. Lysosomes are central to many biological processes, such as antigen presentation, melanocyte function, cytotoxic T cell killing, maintenance of cellular homeostasis, and membrane repair. However, the functions of many lysosomal protein-coding genes are still unknown, and RNAi screening may provide a useful tool to reveal the roles of these genes in specific pathways and processes.
Fig. 1. Gene silencing by RNAi. (Mohr SE, et al., 2014)
Lysosomal Trafficking RNAi Screening Services
CD BioSciences is committed to designing RNAi screens to interrogate lysosomal function. We focus on short interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs) for high-throughput screening analysis of lysosomal protein genes. We formulate detailed RNAi screening programs, including the selection of screening systems, hybrid screening and array screening, and detection development and validation of new gene candidates. Here, our engineers combine multiple strategies to provide an efficient RNAi screening service for lysosomal trafficking.
- We choose the appropriate cell type, and siRNA or shRNA can be easily introduced in a high-throughput manner.
- We combined array and ensemble RNAi screening methods for analysis.
- We performed multiple tests for robustness to ensure reliable data from large-scale array screens, including testing viral titers and run independence for delivery of a given shRNA sequence.
- For established and validated experimental conditions, we evaluate the performance of positive and negative controls.
- Silencing of mRNA transcripts was confirmed by quantitative reverse transcription polymerase chain reaction (RT-PCR).
Advantages of Our Services
- We have extensive experience with arrayed shRNA screens for lysosomal transport in biological processes.
- Combining genome-wide siRNA libraries and flow cytometry.
- Making siRNA or shRNA arrays of lysosomal protein genes available.
- Contributing to lysosomal biology research.
- Genome-scale RNAi screening is applicable to a broad range of organisms.
- Ideal for miniaturization and automation, facilitating high-throughput studies of lysosomal protein genes.
Our RNAi screening services for lysosomal trafficking are well received by customers around the world. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
References
- Garg S, Brenner MB. (2015) RNAi screens of lysosomal trafficking. Methods Cell Biol. 126:119-38.
- Mohr SE, Smith JA, et al. (2014) RNAi screening comes of age: improved techniques and complementary approaches. Nat Rev Mol Cell Biol. 15(9):591-600.