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Vacuolin-1

Cat No.:

LIA001

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Chemical Structure：

Description of Vacuolin-1

Vacuolin-1 is a potent inhibitor of cell-penetrating lysosomal exocytosis. Vacuolin-1 blocks lysosomal Ca²⁺-dependent exocytosis, preventing the release of lysosomal contents without affecting the reclosure process. Vacuolin-1 is a potent and selective PIKfyve inhibitor that inhibits late autophagy by impairing lysosomal maturation.

Product Information of Vacuolin-1

Purity

0.9882

Molecular Weight

577.42

Formula

C₂₆H₂₄IN₇O

CAS Number

351986-85-1

Pack Size

5 mg/10 mg/ 50 mg/100 mg

Storage

Store at -20°C, stored under nitrogen.

Shelf-Life

6 months(stored in solvent).
1 month (stored under nitrogen).

Shipping Conditions

Room temperature in continental US, may vary elsewhere.

In Vitro Studies

Vacuolin-1 (1 μM; Pretreatment 1 hour) blocks ionomycin-induced exocytosis of lysosomes but not of enlargeosomes. It results in vacuolation of lysosomes (A) and complete inhibition of the surface expression of Lamp-1.
Vacuolin-1 (1 μM; 20-180 min) disrupts the segregation of inner and limiting membranes characteristic of endosomes and lysosomes. Ultrastructural analysis also shows that vacuolin-1 favours fusion of inner and limiting membranes.
Vacuolin-1 (5 or 10 μM; 2 hours) blocks the Ca²⁺-dependent release of β-hexosaminidase from lysosomes. In HeLa cells, ionomycin results in the expected release of 18-20% of lysosomal β-hexosaminidase. But when cells pretreated with vacuolin-1, releases no more β-hexosaminidase compared with cells that are not exposed to ionomycin (4%).

For research use only, not intended for any clinical use.