Fluorescent probes locate lysosomes by fluorescent imaging of lysosomes, track their positional changes, and observe their morphological characteristics, so as to study many physiological processes in organisms, such as cholesterol balance, tissue remodeling, etc. CD BioSciences' expertise in lysosomes provides strong support for lysosomal positioning analysis services. Our advanced imaging technology platform will bring you efficient and economical services to ensure your 100% satisfaction.
Introduction of Lysosome Positioning
Lysosomes are widely distributed throughout the cytoplasm. Some lysosomes are relatively stationary, and some lysosomes move prograde and retrograde along microtubule tracks between the center and periphery of the cell. Lysosomal motility is not continuous. In addition, the distribution and motility of lysosomes can change under certain conditions. Various reactive small molecules (e.g., hydrogen ions, reductive species, reactive oxygen species, reactive nitrogen species, metal cations, anions, etc.) in the lysosome are involved in various biochemical reactions within the lysosome, and their concentration and distribution affect a variety of cellular physiological processes. At the same time, the lysosomal microenvironment also affects the function of lysosomes. The study of the active small molecules and microenvironment in lysosomes is important for understanding the molecular mechanisms of lysosomal activities and the diagnosis and treatment of lysosomal-related diseases.
Fig. 1. Multiple functions of lysosomes are influenced by their positioning and motility. (Pu J, et al., 2016)
Our Lysosome Positioning Analysis Services
Fluorescent probes have gained much attention in the study of lysosomes as a powerful tool in the study of subcellular structures. CD BioSciences has designed and synthesized a series of organic small molecule-based lysosome-targeted fluorescent probes to efficiently detect the spatial and temporal distribution of active small molecules and microenvironmental processes in lysosomes. Our engineers have used different strategies to analyze lysosomal localization.
(1) To exploit the acidic physiological environment within the lysosome to develop amine small molecule probes that selectively accumulate in the acidic environment.
(2) To exploit the metabolic function of lysosomes to develop large molecule probes, which generally enter the cell through endocytosis and then go through different cytosolic pathways to reach the lysosome.
(3) Using the characteristic hydrolytic enzymes or membrane proteins of lysosomes to design fluorescent probes for specific recognition of enzymes or proteins.
(4) Using intramolecular charge transfer, photoinduced electron transfer, fluorescence resonance energy transfer, aggregation- induced luminescence, excited state intramolecular proton transfer, and proton excited resonance charge to design probe fluorophores and recognition groups.
Although lysosomes are widely distributed within cells, they can be distinguished spatially based on their location. Our lysosomal localization analysis service is suitable for molecular imaging of lysosomally active small molecules in cells, tissues and organisms. Here, we provide services for analyzing lysosome positioning as follows:
Features of Our Services
- Interacting molecules that identify lysosome positioning and movement.
- Amine probes are not very specific for lysosomal localization and are not suitable for live cell detection.
- Macromolecular fluorescent probes have some toxicity and are not suitable for long-term tracing of lysosomes.
- The preparation of characteristic protein probes is expensive, time-consuming, poorly water-soluble, and storage conditions are strict, making it difficult to popularize.
- It can be used as an effective lysosome-specific fluorescent probe.
Our lysosome positioning analysis service will help scientists to further understand the molecular mechanism of life activities in which lysosomes are involved, as well as the causes and control factors of lysosome-related diseases. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
References
- Pu J, Guardia CM, Keren-Kaplan T, Bonifacino JS. (2016) Mechanisms and functions of lysosome positioning. J Cell Sci. 129(23):4329-4339.
- Korolchuk V I, Saiki S, Lichtenberg M, et al. (2011) Lysosomal positioning coordinates cellular nutrient responses[J]. Nature cell biology. 13(4): 453-460.