Malignancy is a growing health problem that constitutes an increasing mortality rate today. The relationship between lysosomes and cancer has received much attention in recent years. In addition to the degradation of substances, lysosomes play an important role in vital cellular signaling processes and are a focus of cancer research. Cancer cells depend on lysosomal function to upregulate their metabolism by altering the number, localization and activity of lysosomes to meet their needs for cell growth and proliferation. Histone proteases are lysosomal hydrolases that can play a dual role in promoting and inhibiting tumor growth. They have been observed to be upregulated and mislocalized in cancer. In addition, histones B, E and S are all known to cause malignancy in different cancers. These lysosomal changes and dysfunctions have profound effects on cancer cell proliferation, migration and invasion, as well as on the escape of cancer cells from the host immune system.
Fig. 1. The key elements involved in lysosomal biology in cancer. (Fennelly C, et al., 2017)
Lysosomal Function Analysis Services in Cancer
Cancer cells have relatively fragile and larger lysosomes that exhibit increased lysosomal biogenesis. Our scientists try to develop new strategies for lysosomes as drug targets for cancer therapy. Here, CD BioSciences is committed to providing comprehensive services to our clients worldwide to analyze lysosomal changes and dysfunction in cancers, including breast, lung, pancreatic and prostate cancers, etc.
- Cancer-Induced Changes in Lysosomal Function
- Cancer-Induced Changes in Lysosome Composition
- Molecular Changes Involving Lysosomal Integrity in Cancer
Our skilled scientists use genomics, post-genomics, proteomics and other modern analytical techniques, especially combining high-throughput technologies with bioinformatics and systems biology approaches to comprehensively examine specific lysosomal changes in cancer. Our proven organoid models and advanced methodological platform will ensure your 100% satisfaction. Our strategy strategies include:
- Analysis of Lysosomal Proteins in Cancer
We analyze lysosomal histoproteinases, including cysteine histoproteinase, serine histoproteinase and aspartate histoproteinase. In addition, we analyze lysosomal membrane proteins, including lysosomal-associated membrane protein 1 (LAMP-1), LAMP-2 and vesicular H+-ATPase (V-ATPase). - Quantifying Lysosomal Membrane Permeability (LMP)
We offer a variety of methods to quantify LMP, such as detecting the enzymatic activity of lysosomal hydrolases in the cytosol, visualizing LMP by imaging lysosomes using fluorescent dextran tracers, or more recently monitoring LMP by using fluorescent antibody probes against galcetins. - Identification of Transcriptional Regulators of Lysosomal Biogenesis
TFEB is thought to be a major regulator of stress responses during lysosomal biogenesis and autophagy, and there are 500-800 direct TFEB target genes. We identify these genes as potential targets for cancer drug development and as reporter genes for detection of lysosomal inhibitors.
Advantages of Our Services
- Organoid models that mimic the physiological environment.
- Systematic and interdisciplinary approaches, and high-throughput technologies such as genomics, post-genomics, transcriptomics, proteomics and metabolomics.
- Comprehensive, more fine-grained analysis services.
- Provide effective strategies to develop biomarkers of lysosomes in cancer.
- Explore new identified therapeutic targets for lysosomal-related diseases.
- Real-time monitoring and determination of the functional status of lysosomes.
Our professional services for the analysis of lysosomal changes and dysfunction in cancers have been well received by customers. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
Reference
- Fennelly C, Amaravadi RK. (2017) Lysosomal Biology in Cancer. Methods Mol Biol. 1594:293-308.