DNA, proteins, carbohydrates and lipids delivered by lysosomes via autophagy and other endocytic pathways. Like proteins, lipids are components of the lysosomal membrane and play key roles in lysosomal biogenesis and function. CD BioSciences' expertise in lysosomes provides strong support for lysosomal lipidomics services. Our advanced unbiased lipidomics analysis platform will bring you efficient and economical service, ensuring your 100% satisfaction.
Lysosomal Lipidomics
Lipids play important roles in the precise regulation of cellular uptake, synthesis, storage, metabolism, and export, and are critical for cellular and organismal homeostasis. Lipids are constantly circulated and redistributed within the cell. Lysosomes play an important role in this cycle, recruiting lipids to lysosomes through autophagy or endocytosis for their degradation by lysosomal hydrolases. Further produced catabolites are redistributed to various cellular compartments to support essential cellular functions. The lysosomal degradation pathway regulates lipid metabolism in cells, and in turn, lysosomes play a major role in lipid metabolism and maintenance of cellular lipid homeostasis. However, excessive lipid supply can largely impair lysosomal function. Lysosomal lipid storage diseases (LSDs) or lipidosis are a group of inherited disorders characterized by the accumulation of lipids in late endosomes and lysosomes.
Fig. 1. Lysosomal stress-response pathways induced by dietary lipid overload. (Jaishy B, et al., 2016)
About Our Lysosomal Lipidomics Services
In contrast to other cell membranes, restricted lysosomal membranes are not cholesterol-rich and have characteristic lipids, sphingomyelin and bis(monoacylglycerol) phosphate (BMP). In addition, dysregulated lysosomal lipid metabolism is a pathogenic cause of human diseases including metabolic disorders. CD BioSciences combines large-scale mass spectrometry (MS) technology and bioinformatics platform to identify and quantify large amounts of lysosomal lipids. We are committed to providing comprehensive lysosomal lipidomics services to our global customers, focusing on monitoring lysosomal lipid metabolism function and dysregulation. Our lysosomal lipidomics analysis involves multiple processes, including:
(1) Lysosomal lipid extraction and isolation
We use organic solvents to isolate lipids from lysosomal proteins. In addition, we develop single-phase extraction methods to facilitate the complete recovery of lysosomal lipid classes.
(2) Lysosomal lipid detection
We analyze lysosomal lipids by birdshot lipidomics, ionize lipids by soft electrospray ionization (ESI), matrix-assisted laser desorption/ionization (MALDI) or desorption electrospray ionization (DESI), and detect lipids by MS.
(3) Quantification of lysosomal lipids
We identify lysosomal lipid species by MS/MS spectral features, which are quantified based on peak intensity or peak area.
(4) Data analysis strategy
We can comprehensively evaluate lysosomal lipidomics datasets manually or through software platforms.
The lipidomics of total cell lysates affects the spatial distribution of all cellular lipids, and we provide specific lysosomal lipidomics to provide insight into lysosomal lipid metabolism. We can perform a comprehensive lipidomic analysis of isolated lysosomes.
- Quantitative Analysis of Lysosomal Lipidome
- Analysis of Lysosomal Lipidome Changes in Lysosomal Storage Diseases
Advantages of Our Services
- Proven MS technology and bioinformatics platform.
- Accurately identify and quantify each unique lipid metabolite.
- Targeted and non-targeted identification of lysosomal lipid species and their quantification under diseased conditions.
- Lysosomal lipidomics enables a complete characterization of the metabolome.
- Contributes to the development of diagnostic tools, drugs and therapeutic strategies for combating metabolic diseases.
The application of our birdshot lipidomics to lysosomal lipidome research has been well received by customers. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
References
- Jaishy B, Abel E D. (2016) Lipids, lysosomes, and autophagy[J]. Journal of lipid research. 57(9): 1619-1635.
- Thelen AM, Zoncu R. (2017) Emerging Roles for the Lysosome in Lipid Metabolism. Trends Cell Biol. 27(11): 833-850.