Autophagy is a collection of transport systems that move cytoplasmic components into lysosomes (vacuoles) with diverse physiological and pathological roles. Microautophagy refers to the process by which lysosomes directly engulf cytosolic substances through membrane invagination. CD BioSciences' expertise in lysosomes provides strong support for the study of lysosomal biology in microautophagy. Our advanced yeast microautophagy, mammalian microautophagy and plant microautophagy platforms can perform autophagy-lysosomal biological analysis to ensure your 100% satisfaction.
Lysosome and Microautophagy
Microautophagy is one of the three major pathways of the autophagy-lysosome system. Although macroautophagy has been extensively studied, microautophagy has received much less attention. Microautophagy is characterized by being directly wrapped by the vesicle/lysosome membrane to accomplish the separation and uptake of cellular components within the lysosome. Microautophagy has a wide range of functions in biosynthetic transport, metabolic adaptation, organelle remodeling, and quality control. In addition, microautophagy coordinates with and complements other pathways. In the 1990s, systematic screening of yeast mutants took microautophagy research to a new level, identifying a large number of genes associated with microautophagy. Researchers used fluorescence microscopy to stain specific mutants to examine the microautophagy status or function of specific genes. With the accumulation of experimental data, the precise mechanisms controlling microautophagy have received increasing attention.
Fig. 1. Scheme of microautophagy. (Li W, et al., 2012)
Lysosome Biology in Microautophagy Study Services
As a fundamental form of autophagy, microautophagy relies on lysosomal/vesicular degradation processes that are non-selective or selective. CD BioSciences is committed to providing comprehensive research services for lysosomal biology in microautophagy to clients worldwide. We have successfully built yeast cell microautophagy, mammalian cell microautophagy and plant microautophagy platforms to help you analyze changes in microautophagy associated with lysosomes, vesicles or endonucleosomes. We develop reliable assays to specifically measure the rate of microautophagy to reveal the diverse morphological and molecular mechanisms of microautophagy.
- Microautophagy with lysosomal prominence.
- Microautophagy with lysosomal invagination.
- Microautophagy with endosomal invagination.
Classifying microautophagic pathways based on the morphology and location of membrane dynamics above helps us to understand the mechanistic scheme of microautophagy and we can provide provide a better kinetic view and selectivity of microautophagic pathways. Our research services for lysosomal biology in microautophagy include:
- Identification of Microautophagy in Yeast
- Identification of Microautophagy in Mammals
- Assessment of Mammalian Endosomal Microautophagy
- Identification of Microautophagy in Plants
Advantages of Our Services
- Mature yeast cell microautophagy, mammalian cell microautophagy and plant microautophagy platforms.
- Identifies and characterizes the unique molecules and mechanisms involved in each microautophagic process.
- Allows specialized disruption of different microautophagic processes and assessment of their physiological functions.
- Provides an opportunity to develop novel interventional strategies for autophagy and lysosome-related diseases.
Our professional services for the study of lysosomal biology in microautophagy have been well received by customers. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
Reference
- Li W, Li J, Bao J. (2012) Microautophagy: lesser-known self-eating[J]. Cellular and molecular life sciences. 69(7): 1125-1136.