Lysosomes are essential for the normal function of cells. More than 300 proteins have been found to be located at the lysosome, and the lysosomal proteome and interactome is constantly growing. Identification of these proteins can help to study the mechanism of lysosomal storage disease. CD BioSciences' expertise in lysosomes provides strong support for lysosomal proteomics services. Our advanced mass spectrometry (MS)-based proteomics technology platform will bring you efficient and economical service, ensuring your 100% satisfaction.
Lysosomal Proteomics
Lysosomes are degradative organelles present in almost all eukaryotic cells and are responsible for the breakdown of various compounds as well as the recovery of their components. After being transported to lysosomes, cargoes are degraded by more than 60 different luminal hydrolytic enzymes. Since the discovery of lysosomes, the identification of functional proteins in lysosomes has received significant attention. While many lysosomal hydrolases were identified by classical biochemical methods, knowledge of the protein composition of lysosomal membranes has long remained fragmented. In the last decade, the proteome of the lysosomal matrix and membrane has been extensively studied using large-scale MS. Characterization of most hydrolases and cofactors as well as several lysosomal membrane proteins is important to study lysosomal storage disease (LSD) and lysosomal-related disease mechanisms.
Fig. 1. Current draft of the lysosomal proteome. (Muthukottiappan P, et al., 2021)
About Our Lysosomal Proteomics Services
Knowledge of lysosomal components is fundamental to our understanding of their function, where lysosomal proteins are involved in various proteomic studies. CD BioSciences has established a large-scale MS platform to study the proteome of lysosomal matrix and membrane. We are committed to providing comprehensive lysosomalomics services to our global customers and focus on identifying novel lysosomal proteins. Here, our engineers use MS-based proteomics approaches to perform a comprehensive analysis of the lysosomal proteome in two ways.
(1) Analysis of known lysosomal proteins by using a whole proteome covering sufficient depth, for example by performing sample fractionation or by using targeted acquisition methods.
(2) Analysis of all proteins located in/on the lysosome by purifying the lysosome, regardless of their known function. Such as for identifying new lysosomal proteins.
In proteomic studies studying cells or tissues, lysosomal proteins are usually not identified/quantified repeatedly because of their relatively low abundance. Based on our advanced technology platform, we can help you identify, quantify and characterize lysosomal proteins on a large scale. Our goal is to accelerate the study of lysosomal function at the molecular level by identifying the functional characteristics of lysosomal proteins. Our lysosomal proteomics service offerings include, but are not limited to:
- Enrichment of Lysosomal Proteins
- Proteomic Analysis of Lysosomes
- Analysis of Lysosomal Proteome Dynamics
- Analysis of Lysosomal Proteins in Complex Samples
Advantages of Our Services
- Proven MS-based proteomics platform.
- Allows increased coverage of low-abundance lysosomal proteins in highly complex samples.
- Allows analysis of lysosomal proteins in the absence of organelle enrichment.
- Provides a comprehensive view of all lysosomal proteins in a sample, applied to lower sample volumes.
- Allows study of the extent to which lysosomal proteins are affected in the absence of an obvious lysosomal phenotype.
- Lysosomal proteins can be identified at the molecular level from different cell types and tissues.
The application of our MS-based approach to lysosomal proteome research has been well received by customers. Our highly skilled and dedicated scientific staff ensures that the most appropriate method and technology is selected for each specialized lysosomal project. If you have any special requirements about our services, please feel free to contact us. We are looking forward to working together with your attractive projects.
References
- Muthukottiappan P, Winter D. (2021) A proteomic view on lysosomes[J]. Molecular Omics.
- Schröder BA, Wrocklage C, Hasilik A, Saftig P. (2010) The proteome of lysosomes. Proteomics. 10(22):4053-76.